Hotspot

clpP

LSC · 69612-71663 · 2052 bp

Back to Eriosema

Pi 0.000975

MarkerSeek score 58.20

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2052
variable_sites: 2
pi: 0.000975
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	AGAATAATGCTTGCCATCCA	AGCTAATTCTTACGTTTCCACAC	40.0	39.1	55.1	57.1	2137-2139 bp	1.0	40.1
clpP_p2	AGAATAATGCTTGCCATCCA	GCTAATTCTTACGTTTCCACACT	40.0	39.1	55.1	57.1	2136-2138 bp	1.0	40.1
clpP_p3	AGAATAATGCTTGCCATCCA	AAGCTAATTCTTACGTTTCCACAC	40.0	37.5	55.1	57.8	2138-2140 bp	1.0	40.1
clpP_p4	AGAATAATGCTTGCCATCCA	GCTAATTCTTACGTTTCCACAC	40.0	40.9	55.1	55.8	2136-2138 bp	1.0	40.1
clpP_p5	AGAATAATGCTTGCCATCCA	AGCTAATTCTTACGTTTCCACA	40.0	36.4	55.1	55.8	2137-2139 bp	1.0	40.1