Hotspot

psbE-petL

LSC · 64147-65389 · 1243 bp

Back to Eriosema

Pi 0.00242

MarkerSeek score 60.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1243
variable_sites: 3
pi: 0.00242
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	TCAGTCGTATTTTACTCAGACCA	50.0	39.1	57.8	57.0	1360-1361 bp	1.0	47.2
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	TCAGTCGTATTTTACTCAGACCA	55.0	39.1	58.2	57.0	1355-1356 bp	1.0	46.9
psbE-petL_p3	CCTGCAATGAATAGGGAGGG	TCAGTCGTATTTTACTCAGACCA	55.0	39.1	57.7	57.0	1434-1435 bp	1.0	46.2
psbE-petL_p4	TCTCCTGTGCTTCCAGACAT	TCAGTCGTATTTTACTCAGACCA	50.0	39.1	58.4	57.0	1353-1354 bp	1.0	45.7
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	TCAGTCGTATTTTACTCAGACCAA	50.0	37.5	57.8	57.7	1360-1361 bp	1.0	44.3