Hotspot

psbD-trnT-GGU

LSC · 29007-30092 · 1086 bp

Back to Eriosema

Pi 0.000921

MarkerSeek score 59.30

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1086
variable_sites: 1
pi: 0.000921
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	CCGCAACCAGTCATCCATAA	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.0	58.1	1216-1226 bp	1.0	79.3
psbD-trnT-GGU_p2	ATAGACCAGACCAACCCACA	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.0	58.1	1253-1263 bp	1.0	79.2
psbD-trnT-GGU_p3	CCGCAACCAGTCATCCATAA	TCAGTGGTAGAGTAACGCCA	50.0	50.0	58.0	58.1	1219-1229 bp	1.0	79.1
psbD-trnT-GGU_p4	ATAGACCAGACCAACCCACA	TCAGTGGTAGAGTAACGCCA	50.0	50.0	58.0	58.1	1256-1266 bp	1.0	79.1
psbD-trnT-GGU_p5	AGACCAACCCACAAAAACGA	GTGGTAGAGTAACGCCATGG	45.0	55.0	57.9	58.1	1246-1256 bp	1.0	78.5