Hotspot

trnG-GCC-psbZ

LSC · 25001-25722 · 722 bp

Back to Eriosema

Pi 0
MarkerSeek score 59.60
Primer available yes
Label trnG-GCC-psbZ

Feature Metadata

label_name
trnG-GCC-psbZ
feature_type
igs
region
LSC
length_bp
722
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-psbZ_p1 CGTCTTCTCCTTGGCAAAGA CGAAGGTTGGTCGAGTAACA 50.0 50.0 57.8 57.6 865-869 bp 1.0 75.8
trnG-GCC-psbZ_p2 ATAACTAAGTGGGGGCGGAT CGAAGGTTGGTCGAGTAACA 50.0 50.0 58.2 57.6 902-906 bp 1.0 75.6
trnG-GCC-psbZ_p3 TAACTAAGTGGGGGCGGATA CGAAGGTTGGTCGAGTAACA 50.0 50.0 57.5 57.6 901-905 bp 1.0 73.3
trnG-GCC-psbZ_p4 AACTAAGTGGGGGCGGATAG CGAAGGTTGGTCGAGTAACA 55.0 50.0 58.9 57.6 900-904 bp 1.0 70.1
trnG-GCC-psbZ_p5 CGTCTTCTCCTTGGCAAAGA CGAAGGTTGGTCGAGTAACAA 50.0 47.6 57.8 58.3 865-869 bp 1.0 69.2