Hotspot

ycf1

SSC · 126844-132513 · 5670 bp

Back to Eriolarynx

Pi 0.000823

MarkerSeek score 53.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5670
variable_sites: 7
pi: 0.000823
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGTTCTATCAGTCAAATCCTTCA	AGAAGAGTTTCAAAAGAAACCA	34.8	31.8	55.3	54.1	234-261 bp	1.0	45.0
ycf1_p2	ACTTGTTCTATCAGTCAAATCCT	AGAAGAGTTTCAAAAGAAACCA	34.8	31.8	55.3	54.1	237-264 bp	1.0	44.9
ycf1_p3	AGGACTAATGTCTATTTCTGGAGT	AGAAGAGTTTCAAAAGAAACCA	37.5	31.8	56.6	54.1	309-336 bp	1.0	44.7
ycf1_p4	AGGACTAATGTCTATTTCTGGAGT	AGAAGAGTTTCAAAAGAAACCAA	37.5	30.4	56.6	54.9	309-336 bp	1.0	44.7
ycf1_p5	AGGACTAATGTCTATTTCTGGAGTT	AGAAGAGTTTCAAAAGAAACCA	36.0	31.8	57.3	54.1	309-336 bp	1.0	44.7