Hotspot

matK-trnK-UUU

LSC · 3691-4411 · 721 bp

Back to Eriolarynx

Pi 0

MarkerSeek score 47.70

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 721
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	GCGAGCTATCTGGCTGTAAA	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.8	58.2	776-785 bp	1.0	77.8
matK-trnK-UUU_p2	GCGAGCTATCTGGCTGTAAA	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.8	58.2	775-784 bp	1.0	77.8
matK-trnK-UUU_p3	TGTTGTTGCGAGCTATCTGG	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.3	58.2	783-792 bp	1.0	77.4
matK-trnK-UUU_p4	TGTTGTTGCGAGCTATCTGG	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.3	58.2	782-791 bp	1.0	77.4
matK-trnK-UUU_p5	TGTGTTGTTGCGAGCTATCT	ACGGTAGAGTACTCGGCTTT	45.0	50.0	57.5	58.2	784-793 bp	1.0	75.9