primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	matK-trnK-UUU	1	GCGAGCTATCTGGCTGTAAA	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	57.8	58.2	0.0	17.1	0.393488	3691	4411	776-785	1.000000	77.8
matK-trnK-UUU_p2	matK-trnK-UUU	2	GCGAGCTATCTGGCTGTAAA	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	57.8	58.2	0.0	17.1	0.393488	3691	4411	775-784	1.000000	77.8
matK-trnK-UUU_p3	matK-trnK-UUU	3	TGTTGTTGCGAGCTATCTGG	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	58.3	58.2	0.0	17.1	0.442779	3691	4411	783-792	1.000000	77.4
matK-trnK-UUU_p4	matK-trnK-UUU	4	TGTTGTTGCGAGCTATCTGG	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	58.3	58.2	0.0	17.1	0.442779	3691	4411	782-791	1.000000	77.4
matK-trnK-UUU_p5	matK-trnK-UUU	5	TGTGTTGTTGCGAGCTATCT	ACGGTAGAGTACTCGGCTTT	20	20	45.0	50.0	57.5	58.2	0.0	17.1	0.624093	3691	4411	784-793	1.000000	75.9
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	7.8	16.9	0.156387	6262	7487	1314-1316	1.000000	79.1
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6262	7487	1297-1299	1.000000	78.6
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CAACGGATCGTGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	7.8	16.9	0.285346	6262	7487	1315-1317	1.000000	78.0
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	7.8	16.9	0.445953	6262	7487	1312-1314	1.000000	76.7
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6262	7487	1311-1313	1.000000	76.7
rpoC2_p1	rpoC2	1	CTCCAATCGATTGACCTGCA	GGATGATGCCGGAAAGGATT	20	20	50.0	50.0	58.0	58.0	34.2	5.0	0.041537	20424	20543	285-285	1.000000	80.2
rpoC2_p2	rpoC2	2	AGGACTCCGGCCATAACATA	GGATGATGCCGGAAAGGATT	20	20	50.0	50.0	57.9	58.0	2.2	5.0	0.110228	20424	20543	221-221	1.000000	79.8
rpoC2_p3	rpoC2	3	TAGGACTCCGGCCATAACAT	GGATGATGCCGGAAAGGATT	20	20	50.0	50.0	57.9	58.0	2.2	5.0	0.110228	20424	20543	222-222	1.000000	79.8
rpoC2_p4	rpoC2	4	CTCCAATCGATTGACCTGCA	AGTCCTCGGAATGGGATGAT	20	20	50.0	50.0	58.0	57.9	34.2	0.0	0.137649	20424	20543	298-298	1.000000	79.4
rpoC2_p5	rpoC2	5	CTCCAATCGATTGACCTGCA	GTCCTCGGAATGGGATGATG	20	20	50.0	55.0	58.0	57.8	34.2	0.0	0.204298	20424	20543	297-297	1.000000	78.9
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.088172	28910	29582	735-736	1.000000	79.6
trnC-GCA-petN_p2	trnC-GCA-petN	2	GTAGCATTTTGGCGACATGG	TTAAAGCAGCCCAAGCAAGA	20	20	50.0	45.0	57.8	58.0	0.0	0.0	0.199350	28910	29582	788-789	1.000000	78.7
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.273256	28910	29582	729-730	1.000000	78.1
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	CCTAGAGTCCACTCCTTCCC	20	20	50.0	60.0	58.1	58.2	0.0	3.0	0.299057	28910	29582	792-793	1.000000	77.9
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTTCCCCAGTTCAAATCCGG	CTAGAGTCCACTCCTTCCCC	20	20	50.0	60.0	58.1	58.2	0.0	3.0	0.299057	28910	29582	791-792	1.000000	77.9
lhbA-trnG-UCC_p1	lhbA-trnG-UCC	1	TCCTGATGGCTGGTCAAGTA	AATCGAACCCGCATCTTCTC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.107466	37810	38086	438-438	1.000000	79.1
lhbA-trnG-UCC_p2	lhbA-trnG-UCC	2	TCCTGATGGCTGGTCAAGTA	GAATCGAACCCGCATCTTCT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.107466	37810	38086	439-439	1.000000	79.1
lhbA-trnG-UCC_p3	lhbA-trnG-UCC	3	TTTGCTTCTCCTGATGGCTG	AATCGAACCCGCATCTTCTC	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.228396	37810	38086	446-446	1.000000	78.2
lhbA-trnG-UCC_p4	lhbA-trnG-UCC	4	TTTGCTTCTCCTGATGGCTG	GAATCGAACCCGCATCTTCT	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.228396	37810	38086	447-447	1.000000	78.2
lhbA-trnG-UCC_p5	lhbA-trnG-UCC	5	TCCTGATGGCTGGTCAAGTA	GAACCCGCATCTTCTCCTTG	20	20	50.0	55.0	58.0	58.6	0.0	0.0	0.675220	37810	38086	434-434	1.000000	74.6
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	57.9	58.0	4.1	0.0	0.156301	43503	44342	929-1018	1.000000	82.7
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	20	20	50.0	50.0	57.9	58.0	4.1	18.7	0.160416	43503	44342	911-1000	1.000000	82.7
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	CAGAGGCTTGGTTCGATCAA	20	20	50.0	50.0	57.9	57.9	4.1	0.0	0.214484	43503	44342	944-1033	1.000000	82.3
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCTGAGTATT	20	20	50.0	50.0	57.9	58.1	4.1	0.0	0.257251	43503	44342	931-1020	1.000000	81.9
psaA-ycf3_p5	psaA-ycf3	5	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	20	20	50.0	50.0	57.9	58.3	4.1	0.0	0.376276	43503	44342	925-1014	1.000000	81.0
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TTGCTAATCGGGGCTAACAC	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.229964	52841	53954	1253-1255	1.000000	78.4
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GCTAATCGGGGCTAACACTC	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.330117	52841	53954	1251-1253	1.000000	77.6
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.7	57.8	0.0	0.0	0.539129	52841	53954	1256-1258	1.000000	75.9
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TTGCTAATCGGGGCTAACAC	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	58.0	57.5	0.0	0.0	0.573736	52841	53954	1252-1254	1.000000	75.7
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GGCTAACACTCCGGAAATGA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.6	57.8	19.7	0.0	0.593873	52841	53954	1242-1244	1.000000	75.5
trnM-CAU-atpE_p1	trnM-CAU-atpE	1	AGTACTGCTTTCATACGGCG	GGCAAATCTAGCTCTCCGAC	20	20	50.0	55.0	58.1	58.1	4.8	0.0	0.218047	54870	55090	328-333	1.000000	79.7
trnM-CAU-atpE_p2	trnM-CAU-atpE	2	AGTACTGCTTTCATACGGCG	GAGGCAAATCTAGCTCTCCG	20	20	50.0	55.0	58.1	57.9	4.8	0.0	0.224865	54870	55090	330-335	1.000000	79.6
trnM-CAU-atpE_p3	trnM-CAU-atpE	3	AGTACTGCTTTCATACGGCG	AGGCAAATCTAGCTCTCCGA	20	20	50.0	50.0	58.1	58.2	4.8	0.0	0.295644	54870	55090	329-334	1.000000	79.1
trnM-CAU-atpE_p4	trnM-CAU-atpE	4	AGTACTGCTTTCATACGGCG	AAGGCTGAAGGAAGGAGACA	20	20	50.0	50.0	58.1	58.3	4.8	0.0	0.342775	54870	55090	357-362	1.000000	78.6
trnM-CAU-atpE_p5	trnM-CAU-atpE	5	AGTACTGCTTTCATACGGCG	AGGCTGAAGGAAGGAGACAA	20	20	50.0	50.0	58.1	58.3	4.8	0.0	0.342775	54870	55090	356-361	1.000000	78.6
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	GTCGTATCTTGCTCAGACCA	20	20	45.0	50.0	58.0	57.1	0.0	0.0	0.958793	67882	69038	1360-1360	1.000000	72.4
psbE-petL_p2	psbE-petL	2	TGACAAATAACCAACCCGCA	AGCCGCTAATAGAAAACCGA	20	20	45.0	45.0	58.0	57.0	0.0	0.0	0.991034	67882	69038	1314-1314	1.000000	72.2
psbE-petL_p3	psbE-petL	3	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.9	57.1	6.4	0.0	0.992787	67882	69038	1349-1349	1.000000	72.2
psbE-petL_p4	psbE-petL	4	CAACCCGCAATGAATAGGGA	AGCCGCTAATAGAAAACCGA	20	20	50.0	45.0	57.9	57.0	6.4	0.0	1.025029	67882	69038	1303-1303	1.000000	71.9
psbE-petL_p5	psbE-petL	5	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGACCA	20	20	55.0	50.0	58.2	57.1	0.9	0.0	1.139384	67882	69038	1267-1267	1.000000	71.0
petB-petD_p1	petB-petD	1	CCGCTTCTTACTGCCGTATT	TATAGATCCATCCCCCGAGG	20	20	50.0	55.0	58.1	57.2	0.0	0.0	0.912746	79646	80546	1007-1007	1.000000	72.9
petB-petD_p2	petB-petD	2	CCGCTTCTTACTGCCGTATT	TTATAGATCCATCCCCCGAGG	20	21	50.0	52.4	58.1	57.9	0.0	0.0	1.180164	79646	80546	1008-1008	1.000000	70.8
petB-petD_p3	petB-petD	3	TATTGCCGCTTCTTACTGCC	TATAGATCCATCCCCCGAGG	20	20	50.0	55.0	58.3	57.2	0.0	0.0	1.184984	79646	80546	1012-1012	1.000000	70.8
petB-petD_p4	petB-petD	4	ACACACTTTTGTATTGCCGC	TATAGATCCATCCCCCGAGG	20	20	45.0	55.0	57.6	57.2	0.0	0.0	1.264376	79646	80546	1023-1023	1.000000	70.1
petB-petD_p5	petB-petD	5	TATTGCCGCTTCTTACTGCC	TTATAGATCCATCCCCCGAGG	20	21	50.0	52.4	58.3	57.9	0.0	0.0	1.452402	79646	80546	1013-1013	1.000000	68.6
rpl16_p1	rpl16	1	AACATGCGGTTTTGTTTCAT	TCAATCAAGGGAAACATTTCT	20	21	35.0	33.3	55.0	53.3	0.0	4.4	8.763480	84476	85922	1563-1580	1.000000	41.0
rpl16_p2	rpl16	2	AACATGCGGTTTTGTTTCAT	ATCAATCAAGGGAAACATTTCT	20	22	35.0	31.8	55.0	53.9	0.0	4.4	9.112894	84476	85922	1564-1581	1.000000	41.0
rpl16_p3	rpl16	3	AACATGCGGTTTTGTTTCAT	AATCAATCAAGGGAAACATTTCT	20	23	35.0	30.4	55.0	54.7	0.0	4.4	9.303398	84476	85922	1565-1582	1.000000	41.0
rpl16_p4	rpl16	4	ACATGCGGTTTTGTTTCATATCT	TCAATCAAGGGAAACATTTCT	23	21	34.8	33.3	57.4	53.3	0.0	4.4	9.367581	84476	85922	1562-1579	1.000000	41.0
rpl16_p5	rpl16	5	AACATGCGGTTTTGTTTCAT	TCGATTGACCATTTGATATAATTGCT	20	26	35.0	30.8	55.0	57.6	0.0	0.0	9.434248	84476	85922	1511-1528	1.000000	41.0
ycf2_p1	ycf2	1	ATTCCGCCAAGATCTCTTCG	TCGTTCCGTTTGAAGAAAGGA	20	21	50.0	42.9	57.8	57.8	0.0	0.0	1.413556	90743	90892	358-358	1.000000	70.0
ycf2_p2	ycf2	2	TCTCTTCGTTAGTTGGGGGA	TCGTTCCGTTTGAAGAAAGGA	20	21	50.0	42.9	57.7	57.8	0.0	0.0	1.496412	90743	90892	346-346	1.000000	69.4
ycf2_p3	ycf2	3	GCAATTCCGCCAAGATCTCT	TCGTTCCGTTTGAAGAAAGGA	20	21	50.0	42.9	58.3	57.8	0.0	0.0	1.509173	90743	90892	361-361	1.000000	69.3
ycf2_p4	ycf2	4	CCGCCAAGATCTCTTCGTTA	TCGTTCCGTTTGAAGAAAGGA	20	21	50.0	42.9	57.4	57.8	0.0	0.0	1.761548	90743	90892	355-355	1.000000	67.3
ycf2_p5	ycf2	5	ATTCCGCCAAGATCTCTTCG	GGAAGGATCCCAAAGAATCGA	20	21	50.0	47.6	57.8	57.4	0.0	8.6	1.791851	90743	90892	340-340	1.000000	67.1
ycf15-trnL-CAA_p1	ycf15-trnL-CAA	1	AGGAGAAGGAAGGCTGGAAT	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	58.0	58.0	0.0	15.9	0.029469	96867	97214	474-474	1.000000	80.6
ycf15-trnL-CAA_p2	ycf15-trnL-CAA	2	AGGAGAAGGAAGGCTGGAAT	TGAAATGGTAGACACGCGAG	20	20	50.0	50.0	58.0	58.0	0.0	15.9	0.030840	96867	97214	476-476	1.000000	80.6
ycf15-trnL-CAA_p3	ycf15-trnL-CAA	3	GGAGAAGGAAGGCTGGAATG	AAATGGTAGACACGCGAGAC	20	20	55.0	50.0	58.0	58.0	0.0	15.9	0.057719	96867	97214	473-473	1.000000	80.4
ycf15-trnL-CAA_p4	ycf15-trnL-CAA	4	GGAGAAGGAAGGCTGGAATG	TGAAATGGTAGACACGCGAG	20	20	55.0	50.0	58.0	58.0	0.0	15.9	0.059089	96867	97214	475-475	1.000000	80.4
ycf15-trnL-CAA_p5	ycf15-trnL-CAA	5	AGGAGAAGGAAGGCTGGAAT	GCCTTGGTGGTGAAATGGTA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.103767	96867	97214	486-486	1.000000	80.0
ndhF-rpl32_p1	ndhF-rpl32	1	GAATAAGTCCCGCGCCTATT	TGCTACCCAATATCCCTTCCT	20	21	50.0	47.6	57.8	57.9	0.0	0.0	1.240176	115335	116118	950-950	1.000000	70.3
ndhF-rpl32_p2	ndhF-rpl32	2	CGGCGAAAACTTTTTGTTGC	TGCTACCCAATATCCCTTCCT	20	21	45.0	47.6	57.7	57.9	0.0	0.0	1.420326	115335	116118	981-981	1.000000	68.9
ndhF-rpl32_p3	ndhF-rpl32	3	GCGAAAACTTTTTGTTGCCG	TGCTACCCAATATCCCTTCCT	20	21	45.0	47.6	57.7	57.9	3.8	0.0	1.420326	115335	116118	979-979	1.000000	68.9
ndhF-rpl32_p4	ndhF-rpl32	4	GTCCCGCGCCTATTAACATA	TGCTACCCAATATCCCTTCCT	20	21	50.0	47.6	57.6	57.9	0.0	0.0	1.518745	115335	116118	944-944	1.000000	68.1
ndhF-rpl32_p5	ndhF-rpl32	5	GAATAAGTCCCGCGCCTATT	TGATGTCGAAGTGCGTTTTT	20	20	50.0	40.0	57.8	56.6	0.0	0.0	1.574935	115335	116118	899-899	1.000000	67.7
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGGAAGGGATATTGGGTAGCA	TCCTAAGAGCAGCGTGTCTA	21	20	47.6	50.0	57.9	57.9	0.0	0.0	1.199135	116287	117095	953-969	1.000000	72.1
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGAAGGGATATTGGGTAGCA	TCGAACCGAGATGCATTAGC	21	20	47.6	50.0	57.9	58.1	0.0	0.0	1.221916	116287	117095	979-995	1.000000	71.8
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGGAAGGGATATTGGGTAGCA	AGCAGCGTGTCTACCAATTT	21	20	47.6	45.0	57.9	57.8	0.0	0.0	1.259861	116287	117095	946-962	1.000000	71.6
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGAAGGGATATTGGGTAGCA	CCTAAGAGCAGCGTGTCTAC	21	20	47.6	55.0	57.9	57.8	0.0	0.0	1.271152	116287	117095	952-968	1.000000	71.5
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGGAAGGGATATTGGGTAGCA	CTAAGAGCAGCGTGTCTACC	21	20	47.6	55.0	57.9	57.8	0.0	0.0	1.271152	116287	117095	951-967	1.000000	71.5
ycf1_p1	ycf1	1	TGTTCTATCAGTCAAATCCTTCA	AGAAGAGTTTCAAAAGAAACCA	23	22	34.8	31.8	55.3	54.1	0.0	15.2	11.607394	129300	129419	234-261	1.000000	45.0
ycf1_p2	ycf1	2	ACTTGTTCTATCAGTCAAATCCT	AGAAGAGTTTCAAAAGAAACCA	23	22	34.8	31.8	55.3	54.1	0.0	15.2	11.621332	129300	129419	237-264	1.000000	44.9
ycf1_p3	ycf1	3	AGGACTAATGTCTATTTCTGGAGT	AGAAGAGTTTCAAAAGAAACCA	24	22	37.5	31.8	56.6	54.1	0.0	15.2	11.326067	129300	129419	309-336	1.000000	44.7
ycf1_p4	ycf1	4	AGGACTAATGTCTATTTCTGGAGT	AGAAGAGTTTCAAAAGAAACCAA	24	23	37.5	30.4	56.6	54.9	0.0	15.2	11.529874	129300	129419	309-336	1.000000	44.7
ycf1_p5	ycf1	5	AGGACTAATGTCTATTTCTGGAGTT	AGAAGAGTTTCAAAAGAAACCA	25	22	36.0	31.8	57.3	54.1	0.0	15.2	11.686561	129300	129419	309-336	1.000000	44.7
trnL-CAA-ycf15_p1	trnL-CAA-ycf15	1	AAATGGTAGACACGCGAGAC	AGGAGAAGGAAGGCTGGAAT	20	20	50.0	50.0	58.0	58.0	15.9	0.0	0.029469	147272	147619	474-474	1.000000	80.6
trnL-CAA-ycf15_p2	trnL-CAA-ycf15	2	TGAAATGGTAGACACGCGAG	AGGAGAAGGAAGGCTGGAAT	20	20	50.0	50.0	58.0	58.0	15.9	0.0	0.030840	147272	147619	476-476	1.000000	80.6
trnL-CAA-ycf15_p3	trnL-CAA-ycf15	3	AAATGGTAGACACGCGAGAC	GGAGAAGGAAGGCTGGAATG	20	20	50.0	55.0	58.0	58.0	15.9	0.0	0.057719	147272	147619	473-473	1.000000	80.4
trnL-CAA-ycf15_p4	trnL-CAA-ycf15	4	TGAAATGGTAGACACGCGAG	GGAGAAGGAAGGCTGGAATG	20	20	50.0	55.0	58.0	58.0	15.9	0.0	0.059089	147272	147619	475-475	1.000000	80.4
trnL-CAA-ycf15_p5	trnL-CAA-ycf15	5	GCCTTGGTGGTGAAATGGTA	AGGAGAAGGAAGGCTGGAAT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.103767	147272	147619	486-486	1.000000	80.0