Hotspot

trnL-CAA-ndhB

IRb · 88704-89279 · 576 bp

Back to Eriochrysis

Pi 0

MarkerSeek score 58.30

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: IRb
length_bp: 576
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	GTCTCGCGTGTCTACCATTT	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	509-636 bp	1.0	83.5
trnL-CAA-ndhB_p2	CTCGCGTGTCTACCATTTCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	507-634 bp	1.0	83.5
trnL-CAA-ndhB_p3	GCGTGTCTACCATTTCACCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.2	57.9	504-631 bp	1.0	82.0
trnL-CAA-ndhB_p4	GTCTCGCGTGTCTACCATTT	CCCCATTCTTGCAATTGCTC	50.0	50.0	58.0	57.7	518-645 bp	1.0	81.5
trnL-CAA-ndhB_p5	CTCGCGTGTCTACCATTTCA	CCCCATTCTTGCAATTGCTC	50.0	50.0	58.0	57.7	516-643 bp	1.0	81.5