Hotspot

trnI-CAU-ycf15

IRb · 84996-87548 · 2553 bp

Back to Eriochrysis

Pi 0
MarkerSeek score 56.70
Primer available yes
Label trnI-CAU-ycf15

Feature Metadata

label_name
trnI-CAU-ycf15
feature_type
igs
region
IRb
length_bp
2553
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-CAU-ycf15_p1 GTTGGGCGCTTTAACCATTC TTCAGGGCCTATCCGAAAGA 50.0 50.0 58.0 58.1 2691-2696 bp 1.0 79.1
trnI-CAU-ycf15_p2 GTTGGGCGCTTTAACCATTC CTCCGCGGATATCTTTGCTT 50.0 50.0 58.0 58.1 2620-2625 bp 1.0 79.0
trnI-CAU-ycf15_p3 GTTGGGCGCTTTAACCATTC GCGGATATCTTTGCTTCGGA 50.0 50.0 58.0 58.1 2616-2621 bp 1.0 79.0
trnI-CAU-ycf15_p4 GTTGGGCGCTTTAACCATTC TCCGCGGATATCTTTGCTTC 50.0 50.0 58.0 58.1 2619-2624 bp 1.0 79.0
trnI-CAU-ycf15_p5 GAGCCAGTTCCAATAGACCG TTCAGGGCCTATCCGAAAGA 55.0 50.0 58.1 58.1 2765-2770 bp 1.0 78.7