Hotspot

petD-rpoA

LSC · 75957-76173 · 217 bp

Back to Eriochrysis

Pi 0.00461

MarkerSeek score 55.10

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 217
variable_sites: 1
pi: 0.00461
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	ATCGGTACTGCAGTAGCTCT	TGGGACACTCTAGAGAAGCA	50.0	50.0	57.9	57.4	358 bp	1.0	74.9
petD-rpoA_p2	TCGGTACTGCAGTAGCTCTT	TGGGACACTCTAGAGAAGCA	50.0	50.0	58.2	57.4	357 bp	1.0	74.1
petD-rpoA_p3	CCAGTAGCTACGACCGTTTT	TGGGACACTCTAGAGAAGCA	50.0	50.0	57.6	57.4	382 bp	1.0	72.5
petD-rpoA_p4	ATCGGTACTGCAGTAGCTCT	TGGGACACTCTAGAGAAGCAT	50.0	47.6	57.9	57.9	358 bp	1.0	70.9
petD-rpoA_p5	CGGTACTGCAGTAGCTCTTT	TGGGACACTCTAGAGAAGCA	50.0	50.0	57.3	57.4	356 bp	1.0	70.1