Hotspot

trnfM-CAU-trnT-GGU

LSC · 13238-16005 · 2768 bp

Back to Eriochrysis

Pi 0.000361
MarkerSeek score 56.30
Primer available yes
Label trnfM-CAU-trnT-GGU

Feature Metadata

label_name
trnfM-CAU-trnT-GGU
feature_type
igs
region
LSC
length_bp
2768
variable_sites
1
pi
0.000361
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU-trnT-GGU_p1 CCTCAAGGTTATGAGCCTCG ACCACTAAGTGAAAAGCCCT 55.0 45.0 57.8 56.7 2901 bp 1.0 67.4
trnfM-CAU-trnT-GGU_p2 CCTCAAGGTTATGAGCCTCG TGAACCGATGACTTATGCCT 55.0 45.0 57.8 56.6 2871 bp 1.0 67.2
trnfM-CAU-trnT-GGU_p3 CCTCGTGAGCTACCAAACTG ACCACTAAGTGAAAAGCCCT 55.0 45.0 58.3 56.7 2886 bp 1.0 67.0
trnfM-CAU-trnT-GGU_p4 CCTCAAGGTTATGAGCCTCG GACTTCTGTCTTACCAAGGCA 55.0 47.6 57.8 57.6 2929 bp 1.0 67.0
trnfM-CAU-trnT-GGU_p5 CCTCAAGGTTATGAGCCTCG TGACTTCTGTCTTACCAAGGC 55.0 47.6 57.8 57.6 2930 bp 1.0 67.0