Hotspot

rpl32-trnL-UAG

SSC · 107796-108346 · 551 bp

Back to Eriochloa

Pi 0.0279

MarkerSeek score 67.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 551
variable_sites: 23
pi: 0.0279
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CCAAAAGGTTTTTCTGGGCA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.0	57.9	609-626 bp	1.0	76.3
rpl32-trnL-UAG_p2	ACCAAAAGGTTTTTCTGGGC	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.0	57.9	610-627 bp	1.0	76.3
rpl32-trnL-UAG_p3	GGTTTTTCTGGGCAACAAACA	TCCTAAGAGCAGCGTGTCTA	42.9	50.0	58.0	57.9	603-620 bp	1.0	76.2
rpl32-trnL-UAG_p4	CCAAAAGGTTTTTCTGGGCA	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.0	57.8	602-619 bp	1.0	75.9
rpl32-trnL-UAG_p5	CCAAAAGGTTTTTCTGGGCA	GCTTGAGCACTGCTTCCTAA	45.0	50.0	57.0	58.2	623-640 bp	1.0	75.7