Hotspot

ndhF-rpl32

SSC · 106778-107615 · 838 bp

Back to Eriochloa

Pi 0.0175
MarkerSeek score 57.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
838
variable_sites
22
pi
0.0175
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT AATTTCACCATGGCGGCTAA 55.0 45.0 58.1 58.2 1626-1636 bp 1.0 81.4
ndhF-rpl32_p2 ACACATTCCGACAAGTTCCC AATTTCACCATGGCGGCTAA 50.0 45.0 58.1 58.2 2047-2057 bp 1.0 80.8
ndhF-rpl32_p3 CACATTCCGACAAGTTCCCA AATTTCACCATGGCGGCTAA 50.0 45.0 58.1 58.2 2046-2056 bp 1.0 80.8
ndhF-rpl32_p4 CCAATCCCAACATGGCAGTA AATTTCACCATGGCGGCTAA 50.0 45.0 57.9 58.2 1991-2001 bp 1.0 80.6
ndhF-rpl32_p5 AGACGTAAGGGGGTCAATCA AATTTCACCATGGCGGCTAA 50.0 45.0 58.1 58.2 1866-1868 bp 0.666667 65.8