Hotspot

petA-psbJ

LSC · 62066-62971 · 906 bp

Back to Eriochloa

Pi 0.0119

MarkerSeek score 69.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 906
variable_sites: 13
pi: 0.0119
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	ACGGTTCGTATTCTGGATTGG	50.0	47.6	57.9	58.1	824-1043 bp	1.0	75.9
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	GGTTCGTATTCTGGATTGGGT	50.0	47.6	57.9	57.7	822-1041 bp	1.0	74.4
petA-psbJ_p3	GGCCTTTTGTTCTTCTTCGC	CGGTTCGTATTCTGGATTGG	50.0	50.0	57.9	56.3	823-1042 bp	1.0	71.2
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTCT	ACGGTTCGTATTCTGGATTGG	50.0	47.6	58.7	58.1	831-1050 bp	1.0	70.8
petA-psbJ_p5	GGCCTTTTGTTCTTCTTCGC	CGGTTCGTATTCTGGATTGGG	50.0	52.4	57.9	58.8	823-1042 bp	1.0	70.3