Hotspot

rbcL-psaI

LSC · 57915-58768 · 854 bp

Back to Eriochloa

Pi 0.023

MarkerSeek score 60.80

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 854
variable_sites: 29
pi: 0.023
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TTTGATTTCGAGCCGGTAGA	GAGGGTAAGTTAAAATCTGTCA	45.0	36.4	57.0	53.5	922-949 bp	1.0	42.9
rbcL-psaI_p2	GATTTCGAGCCGGTAGATACC	GAGGGTAAGTTAAAATCTGTCA	52.4	36.4	58.1	53.5	919-946 bp	1.0	42.9
rbcL-psaI_p3	ATTTCGAGCCGGTAGATACC	GAGGGTAAGTTAAAATCTGTCA	50.0	36.4	56.9	53.5	918-945 bp	1.0	42.9
rbcL-psaI_p4	CTTGTGAAGTATGGAAGGCGA	GAGGGTAAGTTAAAATCTGTCA	47.6	36.4	58.0	53.5	948-975 bp	1.0	42.8
rbcL-psaI_p5	TTGTGAAGTATGGAAGGCGA	GAGGGTAAGTTAAAATCTGTCA	45.0	36.4	56.9	53.5	947-974 bp	1.0	42.8