Hotspot

psbM-petN

LSC · 18120-18885 · 766 bp

Back to Eriochloa

Pi 0.011

MarkerSeek score 63.70

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 766
variable_sites: 11
pi: 0.011
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	AAAACAGCCAGCCAAAATGA	AGTATGGGGGAGAAGTGGAC	40.0	55.0	56.7	58.1	531-818 bp	1.0	73.9
psbM-petN_p2	AAAACAGCCAGCCAAAATGA	GTATGGGGGAGAAGTGGACT	40.0	55.0	56.7	58.1	530-817 bp	1.0	73.9
psbM-petN_p3	AAAACAGCCAGCCAAAATGA	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.7	58.2	589-876 bp	1.0	73.5
psbM-petN_p4	AAAACAGCCAGCCAAAATGA	TATGGGGGAGAAGTGGACTC	40.0	55.0	56.7	57.8	529-816 bp	1.0	73.4
psbM-petN_p5	AAAACAGCCAGCCAAAATGA	GGGGGAGAAGTGGACTCTAG	40.0	60.0	56.7	58.2	526-813 bp	1.0	73.1