Hotspot

trnfM-CAU-trnG-UCC

LSC · 13119-13210 · 92 bp

Back to Eriochloa

Pi 0.101
MarkerSeek score 68.50
Primer available yes
Label trnfM-CAU-trnG-UCC

Feature Metadata

label_name
trnfM-CAU-trnG-UCC
feature_type
igs
region
LSC
length_bp
92
variable_sites
14
pi
0.101
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU-trnG-UCC_p1 CCTCGTGAGCTACCAAACTG AGCCTTCCAAGCTAACGATG 55.0 50.0 58.3 58.0 168-176 bp 1.0 84.0
trnfM-CAU-trnG-UCC_p2 CCTCAAGGTTATGAGCCTCG AGCCTTCCAAGCTAACGATG 55.0 50.0 57.8 58.0 183-191 bp 1.0 83.9
trnfM-CAU-trnG-UCC_p3 CCTCGTGAGCTACCAAACTG CCTAGCCTTCCAAGCTAACG 55.0 55.0 58.3 58.1 171-179 bp 1.0 83.7
trnfM-CAU-trnG-UCC_p4 CCTCAAGGTTATGAGCCTCG CCTAGCCTTCCAAGCTAACG 55.0 55.0 57.8 58.1 186-194 bp 1.0 83.6
trnfM-CAU-trnG-UCC_p5 CCTCAAGGTTATGAGCCTCG CCAACGTCGACTATAACCCC 55.0 55.0 57.8 57.8 204-212 bp 1.0 81.9