Hotspot

ycf1

SSC · 120994-126108 · 5115 bp

Back to Eriocaulon

Pi 0.0464

MarkerSeek score 75.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5115
variable_sites: 781
pi: 0.0464
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCGTATCAAACCAATAGCGA	TACCAACGTCGTCGAGAATG	42.9	50.0	58.1	57.7	197-254 bp	0.928571	78.3
ycf1_p2	TGCGTATCAAACCAATAGCGA	CTTACCAACGTCGTCGAGAA	42.9	50.0	58.1	57.7	199-256 bp	0.928571	77.7
ycf1_p3	TGCGTATCAAACCAATAGCGA	TCAATTCACAGAGGGGAGGT	42.9	50.0	58.1	58.0	521-578 bp	0.428571	59.8
ycf1_p4	TGCGTATCAAACCAATAGCG	TCAATTCACAGAGGGGAGGT	45.0	50.0	56.6	58.0	521-578 bp	0.428571	57.5
ycf1_p5	CCCTTTTGCGTATCAAACCA	TCAATTCACAGAGGGGAGGT	45.0	50.0	56.6	58.0	527-584 bp	0.428571	57.4