Hotspot

rps15-ycf1

SSC · 120558-120993 · 436 bp

Back to Eriocaulon

Pi 0.076

MarkerSeek score 79.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 436
variable_sites: 99
pi: 0.076
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCCCCTGTGTAAATAGCCCA	TCAATTCACAGAGGGGAGGT	50.0	50.0	58.0	58.0	1515-1595 bp	0.428571	68.6
rps15-ycf1_p2	AAATGAGAAGCGATGCGACT	TCAATTCACAGAGGGGAGGT	45.0	50.0	58.0	58.0	1709-1789 bp	0.428571	68.5
rps15-ycf1_p3	AGAAGCGATGCGACTCAATT	TCAATTCACAGAGGGGAGGT	45.0	50.0	58.0	58.0	1704-1784 bp	0.428571	68.5
rps15-ycf1_p4	ATGATCAGTCGAAGAACGCC	TCAATTCACAGAGGGGAGGT	50.0	50.0	58.1	58.0	1454-1534 bp	0.428571	68.2
rps15-ycf1_p5	GATCAGTCGAAGAACGCCAT	TCAATTCACAGAGGGGAGGT	50.0	50.0	58.1	58.0	1452-1532 bp	0.428571	68.2