Hotspot

psaC-ndhE

SSC · 114256-114818 · 563 bp

Back to Eriocaulon

Pi 0.0741

MarkerSeek score 79.70

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 563
variable_sites: 130
pi: 0.0741
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	TTACACCCGTCCCAAGGTAT	GCAGCTATTGGGTTGGCTAT	50.0	50.0	58.0	58.0	754-791 bp	0.857143	85.8
psaC-ndhE_p2	TCTGATCAGAAAAGCCCGTG	GCAGCTATTGGGTTGGCTAT	50.0	50.0	57.9	58.0	983-1028 bp	0.857143	85.4
psaC-ndhE_p3	ATTTCGAGTCACTGGCCAAG	GCAGCTATTGGGTTGGCTAT	50.0	50.0	58.2	58.0	1355-1395 bp	0.857143	84.7
psaC-ndhE_p4	TAATCGCGCATTTCGAGTCA	GCAGCTATTGGGTTGGCTAT	45.0	50.0	58.1	58.0	1367-1404 bp	0.785714	80.1
psaC-ndhE_p5	AAAAATCTGTTGGGCAAGCG	GCAGCTATTGGGTTGGCTAT	45.0	50.0	57.8	58.0	837-874 bp	0.214286	48.4