Hotspot

psbE-petL

LSC · 62378-63254 · 877 bp

Back to Eriocaulon

Pi 0.0487

MarkerSeek score 84.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 877
variable_sites: 135
pi: 0.0487
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	GCCAGCCATCGTACTGTAAA	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.0	57.9	1681-1710 bp	1.0	91.0
psbE-petL_p2	TGGTCATTCAAGGCCTCCTA	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.0	57.9	1641-1670 bp	1.0	91.0
psbE-petL_p3	GCCAGCCATCGTACTGTAAA	GGGTACAAAATGTCACGGGT	50.0	50.0	58.0	58.1	1878-1907 bp	1.0	90.6
psbE-petL_p4	GCCAGCCATCGTACTGTAAA	TGGGTACAAAATGTCACGGG	50.0	50.0	58.0	58.1	1879-1908 bp	1.0	90.6
psbE-petL_p5	GCCAGCCATCGTACTGTAAA	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.0	58.1	1867-1896 bp	1.0	90.5