Hotspot

petA-psbJ

LSC · 60915-61605 · 691 bp

Back to Eriocaulon

Pi 0.0726

MarkerSeek score 80.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 691
variable_sites: 155
pi: 0.0726
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAAAAGGTTCAGTTGTACGA	TTCCCCTTTGGCTGATAGGT	38.1	50.0	55.4	58.3	783-826 bp	1.0	60.7
petA-psbJ_p2	ACAGTTTGAAAAGGTTCAGTTGT	TTCCCCTTTGGCTGATAGGT	34.8	50.0	57.3	58.3	789-832 bp	1.0	59.7
petA-psbJ_p3	AGGTTCAGTTGTACGAATTGA	TTCCCCTTTGGCTGATAGGT	38.1	50.0	55.2	58.3	778-821 bp	1.0	58.9
petA-psbJ_p4	TGAAAAGGTTCAGTTGTACGAA	TTCCCCTTTGGCTGATAGGT	36.4	50.0	56.2	58.3	783-826 bp	1.0	58.8
petA-psbJ_p5	TTGAAAAGGTTCAGTTGTACGA	TTCCCCTTTGGCTGATAGGT	36.4	50.0	56.2	58.3	784-827 bp	1.0	58.8