Hotspot

ycf4-cemA

LSC · 58380-59018 · 639 bp

Back to Eriocaulon

Pi 0.0635

MarkerSeek score 83.50

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 639
variable_sites: 125
pi: 0.0635
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GGCCTATTTCTTACGTGTACCA	TGATAGAGAGACCCACCAGG	45.5	55.0	57.9	57.5	687-772 bp	1.0	71.3
ycf4-cemA_p2	TGGCCTATTTCTTACGTGTACC	TGATAGAGAGACCCACCAGG	45.5	55.0	57.9	57.5	688-773 bp	1.0	71.3
ycf4-cemA_p3	GGCCTATTTCTTACGTGTACCA	ATGATAGAGAGACCCACCAGG	45.5	52.4	57.9	58.0	688-773 bp	1.0	66.7
ycf4-cemA_p4	TGGCCTATTTCTTACGTGTACC	ATGATAGAGAGACCCACCAGG	45.5	52.4	57.9	58.0	689-774 bp	1.0	66.7
ycf4-cemA_p5	GGCCTATTTCTTACGTGTACC	TGATAGAGAGACCCACCAGG	47.6	55.0	56.2	57.5	687-772 bp	1.0	65.5