Hotspot

ndhC-trnV-UAC

LSC · 48773-49494 · 722 bp

Back to Eriocaulon

Pi 0.0619

MarkerSeek score 84.20

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 722
variable_sites: 147
pi: 0.0619
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TGTCATATTTGTGAAGCAGAAACA	GGTTCGAATCCGTATAGCCC	33.3	55.0	57.4	57.9	577-815 bp	1.0	54.3
ndhC-trnV-UAC_p2	TGTCATATTTGTGAAGCAGAAACA	CGGTTCGAATCCGTATAGCC	33.3	55.0	57.4	58.3	578-816 bp	1.0	52.5
ndhC-trnV-UAC_p3	ATGTCATATTTGTGAAGCAGAAACA	GGTTCGAATCCGTATAGCCC	32.0	55.0	57.8	57.9	578-816 bp	1.0	52.0
ndhC-trnV-UAC_p4	TGTCATATTTGTGAAGCAGAAACAT	GGTTCGAATCCGTATAGCCC	32.0	55.0	57.8	57.9	577-815 bp	1.0	52.0
ndhC-trnV-UAC_p5	GTCATATTTGTGAAGCAGAAACA	GGTTCGAATCCGTATAGCCC	34.8	55.0	55.8	57.9	576-814 bp	1.0	52.0