Hotspot

ycf3-trnS-GCU

LSC · 42758-43339 · 582 bp

Back to Eriocaulon

Pi 0.0544

MarkerSeek score 88.30

Primer available yes

Label ycf3-trnS-GCU

Feature Metadata

label_name: ycf3-trnS-GCU
feature_type: igs
region: LSC
length_bp: 582
variable_sites: 103
pi: 0.0544
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 88.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GCU_p1	AGGTTGTTGGAATGATTTGCA	ATTAGCAATCCGCCGCTTTA	38.1	45.0	56.6	58.0	684-735 bp	1.0	72.5
ycf3-trnS-GCU_p2	AGGTTGTTGGAATGATTTGCA	ACGGAAAGAGAGGGATTCGA	38.1	50.0	56.6	58.2	733-784 bp	1.0	71.6
ycf3-trnS-GCU_p3	AGGTTGTTGGAATGATTTGCAA	ATTAGCAATCCGCCGCTTTA	36.4	45.0	57.3	58.0	684-735 bp	1.0	70.2
ycf3-trnS-GCU_p4	AGGTTGTTGGAATGATTTGCA	GCTTTAGTCCACTCAGCCAT	38.1	50.0	56.6	57.6	670-721 bp	1.0	69.5
ycf3-trnS-GCU_p5	AGGTTGTTGGAATGATTTGCAA	ACGGAAAGAGAGGGATTCGA	36.4	50.0	57.3	58.2	733-784 bp	1.0	69.3