Hotspot

psaA-ycf3

LSC · 40194-40830 · 637 bp

Back to Eriocaulon

Pi 0.0412

MarkerSeek score 81.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 637
variable_sites: 90
pi: 0.0412
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCACTCCTCAAAAGAGGTT	ACAAAGCGTTTTGAATCTTCA	50.0	33.3	57.0	54.9	644-791 bp	1.0	52.0
psaA-ycf3_p2	CTGGCCCACTCCTCAAAAG	ACAAAGCGTTTTGAATCTTCA	57.9	33.3	58.0	54.9	648-795 bp	1.0	52.0
psaA-ycf3_p3	TTTCACTTCTTGTTCCGGCG	ACAAAGCGTTTTGAATCTTCA	50.0	33.3	59.1	54.9	598-745 bp	1.0	52.0
psaA-ycf3_p4	TTTTCACTTCTTGTTCCGGC	ACAAAGCGTTTTGAATCTTCA	45.0	33.3	56.9	54.9	599-746 bp	1.0	52.0
psaA-ycf3_p5	CCCACTCCTCAAAAGAGGTTT	ACAAAGCGTTTTGAATCTTCA	47.6	33.3	57.8	54.9	644-791 bp	1.0	52.0