Hotspot

psbM-trnD-GUC

LSC · 28220-29133 · 914 bp

Back to Eriocaulon

Pi 0.0539

MarkerSeek score 88.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 914
variable_sites: 164
pi: 0.0539
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 88.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TTGGCTAACTGTTTTGACGT	TTCAATTGGTCAGAGCACCG	40.0	50.0	55.8	58.5	664-1120 bp	1.0	70.7
psbM-trnD-GUC_p2	TTTGGCTAACTGTTTTGACGT	TTCAATTGGTCAGAGCACCG	38.1	50.0	56.6	58.5	665-1121 bp	1.0	68.9
psbM-trnD-GUC_p3	TTGGCTAACTGTTTTGACGT	GTTCAATTGGTCAGAGCACC	40.0	50.0	55.8	57.0	665-1121 bp	1.0	66.6
psbM-trnD-GUC_p4	TTTTGGCTAACTGTTTTGACGT	TTCAATTGGTCAGAGCACCG	36.4	50.0	57.3	58.5	666-1122 bp	1.0	66.5
psbM-trnD-GUC_p5	TTTGGCTAACTGTTTTGACGT	GTTCAATTGGTCAGAGCACC	38.1	50.0	56.6	57.0	666-1122 bp	1.0	64.7