Hotspot

petN-psbM

LSC · 27391-28114 · 724 bp

Back to Eriocaulon

Pi 0.0759

MarkerSeek score 86.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 724
variable_sites: 166
pi: 0.0759
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 86.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTATGGGGAAGGAGTGGAC	ACGTCAAAACAGTTAGCCAA	55.0	40.0	58.1	55.8	671-778 bp	0.928571	70.7
petN-psbM_p2	GTATGGGGAAGGAGTGGACT	ACGTCAAAACAGTTAGCCAA	55.0	40.0	58.1	55.8	670-777 bp	0.928571	70.7
petN-psbM_p3	ATGGGGAAGGAGTGGACTTT	ACGTCAAAACAGTTAGCCAA	50.0	40.0	58.2	55.8	668-775 bp	0.928571	69.8
petN-psbM_p4	AGTATGGGGAAGGAGTGGAC	ACGTCAAAACAGTTAGCCAAA	55.0	38.1	58.1	56.6	671-778 bp	0.928571	68.9
petN-psbM_p5	GTATGGGGAAGGAGTGGACT	ACGTCAAAACAGTTAGCCAAA	55.0	38.1	58.1	56.6	670-777 bp	0.928571	68.9