Hotspot

rpoB-trnC-GCA

LSC · 26151-26923 · 773 bp

Back to Eriocaulon

Pi 0.0538

MarkerSeek score 84.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 773
variable_sites: 147
pi: 0.0538
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGAGCCCATATCCTTGCTTT	CCTTACCTCTTGGCCATGTC	50.0	55.0	58.0	58.0	1292-1367 bp	1.0	91.4
rpoB-trnC-GCA_p2	CGAGCCCATATCCTTGCTTT	AAAGGATTTGCAGTCCCCTG	50.0	50.0	58.0	58.1	1312-1387 bp	1.0	91.2
rpoB-trnC-GCA_p3	TTGTTTCCTACTCACGCGAG	CCTTACCTCTTGGCCATGTC	50.0	55.0	57.9	58.0	1308-1383 bp	1.0	91.2
rpoB-trnC-GCA_p4	TTGTTTCCTACTCACGCGAG	AAAGGATTTGCAGTCCCCTG	50.0	50.0	57.9	58.1	1328-1403 bp	1.0	90.9
rpoB-trnC-GCA_p5	TCAAAAATAAGCCTGCCGGT	CCTTACCTCTTGGCCATGTC	45.0	55.0	58.1	58.0	1045-1120 bp	0.928571	88.1