Hotspot

trnS-GGA-trnG-UCC

LSC · 7172-8548 · 1377 bp

Back to Eriocaulon

Pi 0.0731

MarkerSeek score 84.00

Primer available yes

Label trnS-GGA-trnG-UCC

Feature Metadata

label_name: trnS-GGA-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1377
variable_sites: 324
pi: 0.0731
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-trnG-UCC_p1	GTTTGGCAAGCTGCTGTAAG	CATCGTTAGCTTGGAAGGCT	50.0	50.0	57.9	58.0	2044-2153 bp	1.0	91.3
trnS-GGA-trnG-UCC_p2	GTTTGGCAAGCTGCTGTAAG	CGTTAGCTTGGAAGGCTAGG	50.0	55.0	57.9	58.1	2041-2150 bp	1.0	91.1
trnS-GGA-trnG-UCC_p3	AGCTGCTGTAAGTTTTCGCT	CATCGTTAGCTTGGAAGGCT	45.0	50.0	58.1	58.0	2036-2145 bp	1.0	90.8
trnS-GGA-trnG-UCC_p4	GTTTGGCAAGCTGCTGTAAG	CGTTAGAGATTGGGCAGGTC	50.0	55.0	57.9	58.1	2619-2735 bp	0.571429	73.9
trnS-GGA-trnG-UCC_p5	GTTTGGCAAGCTGCTGTAAG	ATTGCGTGCCTACTTACGAG	50.0	50.0	57.9	58.1	2657-2662 bp	0.214286	48.1