Hotspot

trnK-UUU-rps16

LSC · 4014-4611 · 598 bp

Back to Eriocaulon

Pi 0.0784

MarkerSeek score 81.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 598
variable_sites: 152
pi: 0.0784
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCACTTAAAAGCCGAG	AGCCGTCTATCGAATCGTTG	50.0	50.0	58.6	57.9	877-915 bp	1.0	86.5
trnK-UUU-rps16_p2	AGTCGCACTTAAAAGCCGAG	CCGTCTATCGAATCGTTGCA	50.0	50.0	58.6	58.2	875-913 bp	1.0	86.1
trnK-UUU-rps16_p3	AGTCGCACTTAAAAGCCGAG	TCTATCCCAATGAGCCGTCT	50.0	50.0	58.6	57.9	889-927 bp	0.928571	83.9
trnK-UUU-rps16_p4	AGTCGCACTTAAAAGCCGAG	ATCCGTTTTTCTTGAGCCGT	50.0	45.0	58.6	58.1	958-996 bp	0.928571	83.6
trnK-UUU-rps16_p5	TCGCACTTAAAAGCCGAGTA	TCTATCCCAATGAGCCGTCT	45.0	50.0	57.3	57.9	887-925 bp	0.928571	82.6