Hotspot

petA-psbJ

LSC · 66004-66985 · 982 bp

Back to Eriobotrya

Pi 0.00593

MarkerSeek score 70.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 982
variable_sites: 40
pi: 0.00593
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGCTCTTCTTGGCATCTGTT	AGCCGGTATTCTTGTGATCG	45.0	50.0	57.4	57.8	1137-1180 bp	1.0	79.6
petA-psbJ_p2	TTGCTCTTCTTGGCATCTGT	AGCCGGTATTCTTGTGATCG	45.0	50.0	57.4	57.8	1138-1181 bp	1.0	79.6
petA-psbJ_p3	CGTGTCCAAGGTCTTTTGCT	AGCCGGTATTCTTGTGATCG	50.0	50.0	58.7	57.8	1153-1196 bp	1.0	78.6
petA-psbJ_p4	GGTCTTTTGCTCTTCTTGGC	AGCCGGTATTCTTGTGATCG	50.0	50.0	57.3	57.8	1144-1187 bp	1.0	78.4
petA-psbJ_p5	TCCATTACGTGTCCAAGGTC	AGCCGGTATTCTTGTGATCG	50.0	50.0	57.2	57.8	1160-1203 bp	1.0	78.0