Hotspot

trnF-GAA-ndhJ

LSC · 51339-52030 · 692 bp

Back to Eriobotrya

Pi 0.00638

MarkerSeek score 68.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 692
variable_sites: 28
pi: 0.00638
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTGAAACGTATC	55.0	50.0	58.0	58.1	862-875 bp	1.0	85.8
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	826-839 bp	1.0	85.7
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	850-863 bp	1.0	84.8
trnF-GAA-ndhJ_p4	GTCGTGAATGGTCGGGATAG	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	875-888 bp	1.0	84.7
trnF-GAA-ndhJ_p5	GAGGATGATGCGTCGTGAAT	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	886-899 bp	1.0	84.5