Hotspot

trnT-GGU-psbD

LSC · 33768-35128 · 1361 bp

Back to Eriobotrya

Pi 0.00815

MarkerSeek score 64.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1361
variable_sites: 48
pi: 0.00815
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGCCAGACCAACCTACAAAA	55.0	50.0	58.1	58.0	1488-1698 bp	1.0	85.9
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGCCAGACCAACCTACAAAA	50.0	50.0	58.1	58.0	1491-1701 bp	1.0	85.7
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	TAGGCCAGACCAACCTACAA	55.0	50.0	58.1	58.0	1490-1700 bp	1.0	85.7
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	ACCTACAAAAACGAAGCGGT	55.0	45.0	58.1	58.0	1478-1688 bp	1.0	85.7
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	TAGGCCAGACCAACCTACAA	50.0	50.0	58.1	58.0	1493-1703 bp	1.0	85.6