Hotspot

trnE-UUC-trnT-GGU

LSC · 33106-33695 · 590 bp

Back to Eriobotrya

Pi 0.0049

MarkerSeek score 39.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 590
variable_sites: 10
pi: 0.0049
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 39.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	ATTTGGATTCGTCGGGACTG	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	1319-1384 bp	1.0	85.1
trnE-UUC-trnT-GGU_p2	GCGGATCGGCAAATTATTGG	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	811-878 bp	1.0	84.9
trnE-UUC-trnT-GGU_p3	ATTTGGATTCGTCGGGACTG	CCATGGCGTTACTCTACCAC	50.0	55.0	58.0	58.1	1298-1363 bp	1.0	84.7
trnE-UUC-trnT-GGU_p4	CTTCCGTATTGGGATTGGGG	GAACCGATGACTTACGCCTT	55.0	50.0	58.0	58.0	882-949 bp	0.818182	77.5
trnE-UUC-trnT-GGU_p5	CTTCCGTATTGGGATTGGGG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.0	58.1	861-928 bp	0.818182	77.1