Hotspot

psbM-trnD-GUC

LSC · 31133-32378 · 1246 bp

Back to Eriobotrya

Pi 0.00473

MarkerSeek score 69.30

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1246
variable_sites: 30
pi: 0.00473
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TCACCTTGGCTGACTGTTTT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.6	58.5	1378-1412 bp	1.0	78.5
psbM-trnD-GUC_p2	AATCACCTTGGCTGACTGTT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.3	58.5	1380-1414 bp	1.0	76.6
psbM-trnD-GUC_p3	ATCACCTTGGCTGACTGTTT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.3	58.5	1379-1413 bp	1.0	76.6
psbM-trnD-GUC_p4	TCACCTTGGCTGACTGTTTT	GTTCAATTGGTCAGAGCACC	45.0	50.0	57.6	57.0	1379-1413 bp	1.0	74.3
psbM-trnD-GUC_p5	ATCACCTTGGCTGACTGTTTT	TTCAATTGGTCAGAGCACCG	42.9	50.0	58.1	58.5	1379-1413 bp	1.0	73.6