Hotspot

rps19

LSC · 84720-84998 · 279 bp

Back to Erigeron

Pi 0.0116

MarkerSeek score 39.00

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 279
variable_sites: 15
pi: 0.0116
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TCCCATGTTCCCCCATTACT	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.0	58.1	992-1067 bp	1.0	86.9
rps19_p2	TCCCATGTTCCCCCATTACT	AACCCTGTAGACCATCCACA	50.0	50.0	58.0	58.0	1029-1104 bp	1.0	86.8
rps19_p3	TCCCATGTTCCCCCATTACT	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.0	58.0	1091-1166 bp	1.0	86.7
rps19_p4	TCCCATGTTCCCCCATTACT	GCAAAAGAAGGGAAATCGGC	50.0	50.0	58.0	58.0	1209-1284 bp	1.0	86.1
rps19_p5	TCCCATGTTCCCCCATTACT	GATGATGGAACGGGAAGACC	50.0	55.0	58.0	58.0	1547-1611 bp	1.0	84.8