Hotspot

ndhF-ycf1

SSC · 127719-127740 · 22 bp

Back to Erigeron

Pi 0.115

MarkerSeek score 65.80

Primer available yes

Label ndhF-ycf1

Feature Metadata

label_name: ndhF-ycf1
feature_type: igs
region: SSC
length_bp: 22
variable_sites: 12
pi: 0.115
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-ycf1_p1	TGATCCCACCCCTTCTTTCT	TTCTTCCTTCTCCGAGCTCA	50.0	50.0	57.9	58.1	1120-1207 bp	1.0	83.7
ndhF-ycf1_p2	TGATCCCACCCCTTCTTTCT	CTAGCACTGGGTAGACCTCA	50.0	55.0	57.9	57.9	991-1078 bp	1.0	83.7
ndhF-ycf1_p3	TGATCCCACCCCTTCTTTCT	TCGTTGTGGTCGGACTCTAT	50.0	50.0	57.9	58.2	1179-1266 bp	1.0	82.7
ndhF-ycf1_p4	TAAATCGGTAGGGGGAGGAC	CTAGCACTGGGTAGACCTCA	55.0	55.0	57.9	57.9	426-485 bp	0.8	75.7
ndhF-ycf1_p5	TAAATCGGTAGGGGGAGGAC	TTCTTCCTTCTCCGAGCTCA	55.0	50.0	57.9	58.1	555-614 bp	0.8	75.6