Hotspot

rpl32-ndhF

SSC · 124408-125498 · 1091 bp

Back to Erigeron

Pi 0.0156
MarkerSeek score 72.90
Primer available yes
Label rpl32-ndhF

Feature Metadata

label_name
rpl32-ndhF
feature_type
igs
region
SSC
length_bp
1091
variable_sites
76
pi
0.0156
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-ndhF_p1 CAAAGCTTTTAACGATGCCCA GTTGCCGTCGGAAAAAGAAG 42.9 50.0 57.7 58.0 1255-1299 bp 1.0 78.2
rpl32-ndhF_p2 ACAAAGCTTTTAACGATGCCC GTTGCCGTCGGAAAAAGAAG 42.9 50.0 57.7 58.0 1256-1300 bp 1.0 78.2
rpl32-ndhF_p3 CAAAGCTTTTAACGATGCCCA TGCCGTCGGAAAAAGAAGAA 42.9 45.0 57.7 58.0 1253-1297 bp 1.0 78.2
rpl32-ndhF_p4 CAAAGCTTTTAACGATGCCCA TTGCCGTCGGAAAAAGAAGA 42.9 45.0 57.7 58.0 1254-1298 bp 1.0 78.2
rpl32-ndhF_p5 ACAAAGCTTTTAACGATGCCC TGCCGTCGGAAAAAGAAGAA 42.9 45.0 57.7 58.0 1254-1298 bp 1.0 78.1