Hotspot

ndhD-ccsA

SSC · 122208-122481 · 274 bp

Back to Erigeron

Pi 0.0245

MarkerSeek score 68.00

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 274
variable_sites: 20
pi: 0.0245
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	TCGCTATCAGTTGACAAGGT	ACAAATTCCGCAATTGTGGC	45.0	45.0	56.6	58.2	437-467 bp	1.0	73.2
ndhD-ccsA_p2	TCCCGATTTTGTTCTTTCGC	ACAAATTCCGCAATTGTGGC	45.0	45.0	57.0	58.2	461-483 bp	0.933333	72.0
ndhD-ccsA_p3	CCCGATTTTGTTCTTTCGCT	ACAAATTCCGCAATTGTGGC	45.0	45.0	57.0	58.2	460-482 bp	0.933333	71.9
ndhD-ccsA_p4	ATCCCGATTTTGTTCTTTCGC	ACAAATTCCGCAATTGTGGC	42.9	45.0	57.5	58.2	462-484 bp	0.933333	67.9
ndhD-ccsA_p5	TCCCGATTTTGTTCTTTCGCT	ACAAATTCCGCAATTGTGGC	42.9	45.0	58.5	58.2	461-483 bp	0.933333	67.8