Hotspot

clpP-psbB

LSC · 72424-72993 · 570 bp

Back to Erigeron

Pi 0.0126

MarkerSeek score 63.00

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 570
variable_sites: 19
pi: 0.0126
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	GTTGCATCTTCCTCTCCAGG	CGATACCAAGGCAAACCCAT	55.0	50.0	58.0	58.2	611-743 bp	1.0	84.2
clpP-psbB_p2	GTTGCATCTTCCTCTCCAGG	AGCAACCAAAGCTGTATGCA	55.0	45.0	58.0	58.4	684-816 bp	1.0	82.8
clpP-psbB_p3	ACCCAAGTTGCATCTTCCTC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	617-749 bp	1.0	82.8
clpP-psbB_p4	GTTGCATCTTCCTCTCCAGG	ACACGATACCAAGGCAAACC	55.0	50.0	58.0	58.5	614-746 bp	1.0	82.3
clpP-psbB_p5	TCCTCTCCAGGACTTCGAAA	CGATACCAAGGCAAACCCAT	50.0	50.0	57.7	58.2	602-734 bp	1.0	82.0