Hotspot

accD-psaI

LSC · 58710-59663 · 954 bp

Back to Erigeron

Pi 0.0101

MarkerSeek score 71.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 954
variable_sites: 45
pi: 0.0101
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAAGGCGTTCTGAGTGAGT	AAAGAAGCCATTGCGATTGC	45.0	45.0	57.4	58.0	993-1143 bp	1.0	82.4
accD-psaI_p2	AAAGGCGTTCTGAGTGAGTT	AAAGAAGCCATTGCGATTGC	45.0	45.0	57.4	58.0	992-1142 bp	1.0	82.4
accD-psaI_p3	AAAAGGCGTTCTGAGTGAGT	TTGCGATTGCCGGAAATACT	45.0	45.0	57.4	58.3	983-1133 bp	1.0	80.4
accD-psaI_p4	AAAGGCGTTCTGAGTGAGTT	TTGCGATTGCCGGAAATACT	45.0	45.0	57.4	58.3	982-1132 bp	1.0	80.4
accD-psaI_p5	AAAGGCGTTCTGAGTGAGTT	AGGCCTACTAAAGGGACCAA	45.0	50.0	57.4	57.6	962-1112 bp	1.0	79.7