Hotspot

ndhC-trnV-UAC

LSC · 50805-51492 · 688 bp

Back to Erigeron

Pi 0.0153

MarkerSeek score 79.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 58
pi: 0.0153
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GTCCTTTGCTAATCGGAGCT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	826-948 bp	1.0	85.2
ndhC-trnV-UAC_p2	CGGTCCTTTGCTAATCGGAG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.4	57.8	828-950 bp	1.0	84.3
ndhC-trnV-UAC_p3	CTCCGGTCCTTTGCTAATCG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.4	57.8	831-953 bp	1.0	84.3
ndhC-trnV-UAC_p4	GTCCTTTGCTAATCGGAGCT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.7	57.5	825-947 bp	1.0	82.4
ndhC-trnV-UAC_p5	GGTCCTTTGCTAATCGGAGC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.7	57.8	827-949 bp	1.0	82.1