Hotspot

ycf3-trnS-GGA

LSC · 44505-45376 · 872 bp

Back to Erigeron

Pi 0.00627

MarkerSeek score 64.30

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 872
variable_sites: 26
pi: 0.00627
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TGCCTCTTTTTCTCCTGAAGT	GGATTCGAACCCTCGGTAAG	42.9	55.0	57.1	57.8	912-1059 bp	1.0	69.4
ycf3-trnS-GGA_p2	TGCCTCTTTTTCTCCTGAAGT	CCTCGGTAAGCAAAAGCCTA	42.9	50.0	57.1	57.6	902-1049 bp	1.0	68.0
ycf3-trnS-GGA_p3	TGCCTCTTTTTCTCCTGAAGT	GATTCGAACCCTCGGTAAGC	42.9	55.0	57.1	58.4	911-1058 bp	1.0	67.7
ycf3-trnS-GGA_p4	TGCCTCTTTTTCTCCTGAAGT	CGAACCCTCGGTAAGCAAAA	42.9	50.0	57.1	58.5	907-1054 bp	1.0	67.3
ycf3-trnS-GGA_p5	TGCCTCTTTTTCTCCTGAAGTT	GGATTCGAACCCTCGGTAAG	40.9	55.0	57.8	57.8	912-1059 bp	1.0	66.9