Hotspot

trnG-UCC-trnfM-CAU

LSC · 36374-36617 · 244 bp

Back to Erigeron

Pi 0.0165

MarkerSeek score 65.70

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 244
variable_sites: 19
pi: 0.0165
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	279-400 bp	1.0	87.0
trnG-UCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.8	58.0	315-436 bp	1.0	86.6
trnG-UCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.8	58.1	273-394 bp	1.0	86.5
trnG-UCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	TTCAGTTCTTCTCTTCGGCG	50.0	50.0	57.8	57.9	337-458 bp	1.0	86.0
trnG-UCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	TCACGGGTTCAAATCCTGTC	50.0	50.0	57.8	57.8	272-393 bp	1.0	85.9