Hotspot

trnS-trnC-GCA

LSC · 8572-9349 · 778 bp

Back to Erigeron

Pi 0.0113

MarkerSeek score 60.00

Primer available yes

Label trnS-trnC-GCA

Feature Metadata

label_name: trnS-trnC-GCA
feature_type: igs
region: LSC
length_bp: 778
variable_sites: 37
pi: 0.0113
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-trnC-GCA_p1	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	924-969 bp	1.0	81.8
trnS-trnC-GCA_p2	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	58.4	906-951 bp	1.0	79.7
trnS-trnC-GCA_p3	CTTTCGTCCACTCAGCCATC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.6	58.1	860-905 bp	1.0	78.2
trnS-trnC-GCA_p4	AACGGAAAGAGAGGGATTCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.3	58.1	925-970 bp	1.0	77.6
trnS-trnC-GCA_p5	CTTTCGTCCACTCAGCCATC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	58.6	58.4	842-887 bp	1.0	76.1