Hotspot

trnK-rps16

LSC · 4243-5096 · 854 bp

Back to Erigeron

Pi 0.00777

MarkerSeek score 68.10

Primer available yes

Label trnK-rps16

Feature Metadata

label_name: trnK-rps16
feature_type: igs
region: LSC
length_bp: 854
variable_sites: 32
pi: 0.00777
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	TCCTAGCCGCACTTAAAAGC	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.3	58.3	963-1004 bp	1.0	80.9
trnK-rps16_p2	CCGCACTTAAAAGCCGAGTA	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.3	58.3	957-998 bp	1.0	80.8
trnK-rps16_p3	TCGCGGTCTTCCAAACTTTA	TTCCTTGAAAAAGGCGCTCA	45.0	45.0	57.5	58.3	1029-1070 bp	1.0	78.5
trnK-rps16_p4	CGCGGTCTTCCAAACTTTAC	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	57.4	58.3	1028-1069 bp	1.0	78.0
trnK-rps16_p5	TCCTAGCCGCACTTAAAAGC	TCCTTGAAAAAGGCGCTCAG	50.0	50.0	58.3	58.8	962-1003 bp	1.0	77.3