Hotspot

ndhF-rpl32

SSC · 103374-104285 · 912 bp

Back to Eriachne

Pi 0.0174

MarkerSeek score 69.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 912
variable_sites: 88
pi: 0.0174
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACACATTCCCACAAGTTCCC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	1148-2069 bp	1.0	89.5
ndhF-rpl32_p2	CACATTCCCACAAGTTCCCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	1147-2068 bp	1.0	89.5
ndhF-rpl32_p3	ACACATTCCCACAAGTTCCC	TCGTTGCCGCTAGAAAATGA	50.0	45.0	58.0	57.9	1151-1543 bp	1.0	89.2
ndhF-rpl32_p4	CACATTCCCACAAGTTCCCA	TCGTTGCCGCTAGAAAATGA	50.0	45.0	58.0	57.9	1150-1542 bp	1.0	89.2
ndhF-rpl32_p5	GAAGAGGGAATTGTGCGGAT	TCGTTGCCGCTAGAAAATGA	50.0	45.0	58.0	57.9	1419-1811 bp	0.826087	81.1