Hotspot

rbcL-psaI

LSC · 56104-56966 · 863 bp

Back to Eriachne

Pi 0.0236

MarkerSeek score 73.50

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 863
variable_sites: 101
pi: 0.0236
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	GATCATATCCACGCCGGTAC	CCAAGATAACTGGAAGCCCC	55.0	55.0	58.0	57.9	1874-1947 bp	1.0	90.0
rbcL-psaI_p2	GATCATATCCACGCCGGTAC	TCACTATACTCGGCTACGCA	55.0	50.0	58.0	58.0	1604-1642 bp	0.608696	72.6
rbcL-psaI_p3	ATATCCACGCCGGTACAGTA	TCACTATACTCGGCTACGCA	50.0	50.0	58.0	58.0	1600-1638 bp	0.608696	72.6
rbcL-psaI_p4	TCTCATTATTGCCGCGACAA	TCACTATACTCGGCTACGCA	45.0	50.0	58.0	58.0	1733-1771 bp	0.608696	72.0
rbcL-psaI_p5	TTGTCTCATTATTGCCGCGA	TCACTATACTCGGCTACGCA	45.0	50.0	58.0	58.0	1736-1774 bp	0.608696	72.0