Hotspot

atpB-rbcL

LSC · 53936-54660 · 725 bp

Back to Eriachne

Pi 0.0267

MarkerSeek score 72.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 725
variable_sites: 71
pi: 0.0267
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CAATTGTAGAAACCCCGGGA	AGTTTCTGTTTGTGGTGACA	50.0	40.0	57.5	55.3	792-825 bp	1.0	64.2
atpB-rbcL_p2	TCAATTGTAGAAACCCCGGG	AGTTTCTGTTTGTGGTGACA	50.0	40.0	57.5	55.3	793-826 bp	1.0	64.2
atpB-rbcL_p3	AATTGTAGAAACCCCGGGAC	AGTTTCTGTTTGTGGTGACA	50.0	40.0	57.5	55.3	791-824 bp	1.0	64.2
atpB-rbcL_p4	GGGACGCGAAGTAGTAGGAT	AGTTTCTGTTTGTGGTGACA	55.0	40.0	58.7	55.3	776-809 bp	1.0	62.9
atpB-rbcL_p5	CAATTGTAGAAACCCCGGGA	TGCTTTAGTTTCTGTTTGTGGTG	50.0	39.1	57.5	58.0	798-831 bp	1.0	61.4